Cis-dichloro-diammine platinum (II) as stain for electron microscopic preparations.

Heinen, Ernst

doi:10.1007/BF00567230

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Article (Scientific journals)

Cis-dichloro-diammine platinum (II) as stain for electron microscopic preparations.

Heinen, Ernst

1977 • In Histochemistry, 51 (2-3), p. 257-60

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https://hdl.handle.net/2268/23200

DOI
10.1007/BF00567230

PubMed
66223

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Keywords :

Animals; Carcinoma, Ehrlich Tumor/ultrastructure; Cisplatin; Microscopy, Electron; Nucleic Acids/analysis; Staining and Labeling

Abstract :

[en] Cis-dichloro-diammine platinum (II) provides a strong contrast to electron microscopic preparations by reacting with nucleic acids and proteins. This staining technique is easy and applicable to ultrathin sections embedded in Epon; various staining conditions have been tested.

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology

Author, co-author :

Heinen, Ernst ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine

Language :

English

Title :

Cis-dichloro-diammine platinum (II) as stain for electron microscopic preparations.

Publication date :

1977

Journal title :

Histochemistry

ISSN :

0301-5564

Publisher :

Springer Verlag, Berlin, Germany

Volume :

Issue :

2-3

Pages :

257-60

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 30 June 2011

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Bibliography

Aggarwal S.K., Wagner R.W., McAllister P.K., Rosenberg B. (1973) Cell-surface-associated nucleic acid in tumorigenic cells make visible with platinum-pyrimidine complexes by electron microscopy. Proc. nat. Acad. Sci. (Wash.) 12:928-932.
Davidson J.P., Faber P.J., Fischer R.G., Mansy S., Peresie H.J., Rosenberg B., Van Camp L. (1975) Platinum-pyrimidine blues and related complexes. A new class of antitumor agents. Cancer Chemother. Rep. 59:287-300.
Millard M.M., Macquet J.P., Theophanides T. (1975) X-ray photoelectron spectroscopy of DNA-Pt complexes. Evidence of O⁶(Gua). N⁷(Gua) chelation of DNA with cis-dichloro-diammine platinum (II). Biochim. Biophys. Acta (Amst.) 402:166-170.
Robins A.B. (1974) Interactions with Biomolecules. Platinum coordination complexes in cancer chemotherapy , T.A., Connors, J.J., Roberts, Springer, Berlin-Heidelberg-New York; 63-78.
Thompson A.J. (1974) The interactions of platinum compounds with biological molecules. Platinum coordination complexes in cancer chemotherapy , T.A., Connors, J.J., Roberts, Springer, Berlin-Heidelberg-New York; 38-62.
Wagner R.W., Rosenberg B., Aggarwal S.K. (1974) Platinum compounds as potential electron microscope stains: compounds containing uracil and thymine. Fed. Proc. 33:1385.

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