Soil sterilization, pathogen and antagonist concentration affect biological control of Fusarium wilt of cape gooseberry by Bacillus velezensis Bs006

Moreno-Velandia, C. A.; Izquierdo-García, L. F.; Ongena, Marc; Kloepper, J. W.; Cotes, A. M.

doi:10.1007/s11104-018-3866-4

Article (Scientific journals)

Soil sterilization, pathogen and antagonist concentration affect biological control of Fusarium wilt of cape gooseberry by Bacillus velezensis Bs006

Moreno-Velandia, C. A.; Izquierdo-García, L. F.; Ongena, Marc et al.

2019 • In Plant and Soil

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/232455

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10.1007/s11104-018-3866-4

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Keywords :

Biological control; Dose-response relationship; Golden berry; Vascular wilting

Abstract :

[en] Background and aim: Fusarium wilt (FW) is the major constraint on cape gooseberry (Physalis peruviana L.) production. Fungicides have been ineffective in disease control and alternative tools are not available. Bacillus velezensis (formerly Bacillus amyloliquefaciens) strain Bs006 has an antagonistic potential against Fusarium oxysporum f. sp. physali (Foph). However, results of in vivo tests have been variable. We examined the effect of biotic sources of variability on the biocontrol activity of Bs006. Methods: Pot experiments in greenhouse were carried out to determine the influence of soil sterilization and concentration of both pathogen and antagonist in soil on biocontrol activity and the effect of pathogen on plant growth promotion by Bs006. Results: Efficacy of Bs006 against FW was significantly lower under sterile than non-sterile soil condition. Diluted liquid culture of Bs006 at 1 × 106 and 1 × 107 cfu.mL−1 reduced FW by up to 97% under low Foph inoculum pressure (102 to 104 cfu.g−1 of soil) but at 1 × 108 cfu.mL−1 biological treatment significantly reduced FW only when the concentration of Foph was 1 × 104 cfu.g−1 by 71%. The evaluation of biomass of Bs006 (1 × 108 cfu.mL−1) and supernatant free of bacteria added at 10% allowed to observe that the supernatant was an additional source of biocontrol variability, since high volumes of supernatant favored the development of the disease. Plant growth promoting activity by Bs006 was reduced by the presence of Foph in the soil. Bs006 grew endophytically in cape gooseberry and had high population levels in the rhizosphere inoculated with Foph. Conclusions: The efficacy of Bs006 to reduce FW was affected by soil sterilization, the concentration of both antagonist and pathogen, and high volumes of supernatant. This work has practical implications for the design of control strategies based on B. velezensis Bs006. © 2018, The Author(s).

Disciplines :

Microbiology

Author, co-author :

Moreno-Velandia, C. A.; Corporación Colombiana de Investigación Agropecuaria – AGROSAVIA, Centro de Investigación Tibaitatá, Kilómetro 14 vía Mosquera-Bogotá, Cundinamarca, Colombia

Izquierdo-García, L. F.; Corporación Colombiana de Investigación Agropecuaria – AGROSAVIA, Centro de Investigación Tibaitatá, Kilómetro 14 vía Mosquera-Bogotá, Cundinamarca, Colombia

Ongena, Marc ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies

Kloepper, J. W.; Department of Entomology and Plant Pathology, Auburn University, Auburn, AL 36849, United States

Cotes, A. M.; Corporación Colombiana de Investigación Agropecuaria – AGROSAVIA, Centro de Investigación Tibaitatá, Kilómetro 14 vía Mosquera-Bogotá, Cundinamarca, Colombia

Language :

English

Title :

Soil sterilization, pathogen and antagonist concentration affect biological control of Fusarium wilt of cape gooseberry by Bacillus velezensis Bs006

Publication date :

2019

Journal title :

Plant and Soil

ISSN :

0032-079X

eISSN :

1573-5036

Publisher :

Springer International Publishing

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 01 February 2019

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