The taxonomy of the Trichophyton rubrum complex: a phylogenomic approach

[en] The medically relevant Trichophyton rubrum species complex has a variety of phenotypic presentations but shows relatively little genetic differences. Conventional barcodes, such as the internal transcribed spacer (ITS) region or the beta-tubulin gene, are not able to completely resolve the relationships between these closely related taxa. T. rubrum, T. soudanense and T. violaceum are currently accepted as separate species. However, the status of certain variants, including the T. rubrum morphotypes megninii and kuryangei and the T. violaceum morphotype yaoundei, remains to be deciphered. We conducted the first phylogenomic analysis of the T. rubrum species complex by studying 3105 core genes of 18 new strains from the BCCM/IHEM culture collection and nine publicly available genomes. Our analyses revealed a highly resolved phylogenomic tree with six separate clades. Trichophyton rubrum, T. violaceum and T. soudanense were confirmed in their status of species. The morphotypes T. megninii, T. kuryangei and T. yaoundei all grouped in their own respective clade with high support, suggesting that these morphotypes should be reinstituted to the species-level. Robinson-Foulds distance analyses showed that a combination of two markers (a ubiquitin-protein transferase and a MYB DNA-binding domain-containing protein) can mirror the phylogeny obtained using genomic data, and thus represent potential new markers to accurately distinguish the species belonging to the T. rubrum complex.

Research center :

Sciensano
InBios - Integrative Biological Sciences - ULiège

Disciplines :

Microbiology

Author, co-author :

Cornet, Luc ; Sciensano > BCCM/IHEM, Mycology and Aerobiology

D'Hooge, Elizabet; Sciensano

Magain, Nicolas ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Biologie de l'évolution et gestion de la biodiversité

Stubbe, Dirk; Sciensano

Packeu, Ann; Sciensano

Baurain, Denis ^✱; Université de Liège - ULiège > Département des sciences de la vie > Phylogénomique des eucaryotes

Becker, Pierre ^✱; Sciensano

^✱ These authors have contributed equally to this work.

Language :

English

Title :

The taxonomy of the Trichophyton rubrum complex: a phylogenomic approach

Publication date :

November 2021

Journal title :

Microbial Genomics

eISSN :

2057-5858

Publisher :

Microbiology Society, London, United Kingdom

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

Politique Scientifique Fédérale (Belgique) - BELSPO
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]

Available on ORBi :

since 10 November 2021

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