A δ-cell subpopulation with pro-β cell identity contributes to efficient age-independent recovery in a zebrafish diabetes model.

Carril Pardo, Claudio Andrès; Massoz, Laura; Dupont, Marie; Bergemann, David; Bourdouxhe, Jordane; Lavergne, Arnaud; Tarifeño Saldivia, Estefania; Helker, Christian Sm; Stainier, Didier Yr True; Peers, Bernard; Voz, Marianne; Manfroid, Isabelle

doi:10.7554/eLife.67576

Article (Scientific journals)

A δ-cell subpopulation with pro-β cell identity contributes to efficient age-independent recovery in a zebrafish diabetes model.

Carril Pardo, Claudio Andrès; Massoz, Laura; Dupont, Marie et al.

2022 • In eLife, 11

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/268196

DOI
10.7554/eLife.67576

PubMed
35060900

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Keywords :

cell biology; zebrafish

Abstract :

[en] Restoring damaged b-cells in diabetic patients by harnessing the plasticity of other pancreatic cells raises the questions of the efficiency of the process and of the functionality of the new Insulin-expressing cells. To overcome the weak regenerative capacity of mammals, we used regeneration-prone zebrafish to study b-cells arising following destruction. We show that most new insulin cells differ from the original b-cells as they coexpress Somatostatin and Insulin. These bihormonal cells are abundant, functional and able to normalize glycemia. Their formation in response to b-cell destruction is fast, efficient and age-independent. Bihormonal cells are transcriptionally close to a subset of d-cells that we identified in control islets and which are characterized by the expression of somatostatin 1.1 (sst1.1) and by genes essential for glucose-induced Insulin secretion in β-cells such as pdx1, slc2a2 and gck. We observed in vivo the conversion of monohormonal sst1.1-expressing cells to sst1.1+ ins+ bihormonal cells following b-cell destruction. Our findings support the conclusion that sst1.1 d-cells possess a pro-b identity enabling them to contribute to the neogenesis of Insulin-producing cells during regeneration. This work unveils that abundant and functional bihormonal cells benefit to diabetes recovery in zebrafish.

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology

Author, co-author :

Carril Pardo, Claudio Andrès ; Université de Liège - ULiège > GIGA

Massoz, Laura ; Université de Liège - ULiège > GIGA Stem Cells - Zebrafish Dev. & Disease Model

Dupont, Marie ; Université de Liège - ULiège > GIGA Stem Cells - Zebrafish Dev. & Disease Model

Bergemann, David

Bourdouxhe, Jordane

Lavergne, Arnaud ; Université de Liège - ULiège > GIGA Platform Genomics

Tarifeño Saldivia, Estefania

Helker, Christian Sm

Stainier, Didier Yr True

Peers, Bernard ; Université de Liège - ULiège > Département des sciences de la vie > GIGA Stem Cells - Zebrafish Dev. & Disease Model

Voz, Marianne ; Université de Liège - ULiège > GIGA Stem Cells - Zebrafish Dev. & Disease Model

Manfroid, Isabelle ; Université de Liège - ULiège > GIGA Stem Cells - Zebrafish Dev. & Disease Model

Language :

English

Title :

A δ-cell subpopulation with pro-β cell identity contributes to efficient age-independent recovery in a zebrafish diabetes model.

Publication date :

2022

Journal title :

eLife

eISSN :

2050-084X

Publisher :

eLife Sciences Publications, United Kingdom

Volume :

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Available on ORBi :

since 11 February 2022

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