[en] The current study has documented changes in the ultrastructure as well as in the intranucleolar distribution of rDNA and rRNA in RT4 (human transitional cell bladder carcinoma) cell nucleoli following a 3-h exposure to toxic doses of 50 microM ametantrone (AMT), 200 microM poly (adenylate-uridylate) (poly r(A-U) or an AMT/poly r(A-U) combination with an AMT/polyribonucleotide ratio of 1:4 and a poly r(A-U) concentration of 200 microM. While the main nucleolar components (fibrillar center (F), dense fibrillar component (D), granular component (G) and interstices (I) can be discerned following all treatments, the nucleoli exhibit: compaction, segregation, a decrease in the number of F, an increase in the size of remaining F, margination of intranucleolar chromatin and retention of intranucleolar pre-rRNA and rRNA. The relative abilities of the test agents to induce nucleolar compaction are AMT/poly r(A-U) > poly r(A-U) > AMT > sham-treated, while the abilities of the test agents to induce the remaining nucleolar changes are AMT/poly r(A-U) > or = AMT > poly r(A-U) > sham-treated cells. Poly r(A-U) and the induced interferon induce nucleolar compaction, while AMT produces nucleolar segregation. These results are consistent with a model in which the poly r(A-U) and/or the AMT inhibit DNA transcription and rRNA processing as well as the release of nascent preribosomes from the nucleolus.
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Altmann, G. and Leblond, C. 1982. Changes in the size and the structure of the nucleoli of columnar cells during their migration from the crypt base to the villus top in rat jejunum. J. Cell Sci., 56, 83-99
Busch, H. and Smetana, K. 1970. The nucleolus, Academic Press, New York
Chapekar, M.S., Knode, M.C. and Galzer, R.I. 1988. The epidermal growth factor- and interferon-independent effect of double-stranded RNA in A431 cells. Mol. Pharmacol. 34, 461-466
Chegini, N. and Safa, A.R. 1987. Influence of mitoxantrone on nucleolar function in MDA-MB-231 human breast tumor cell line. Cancer Lett. 37, 327-336.
Chegini, N., Safa, A.R. and Tseng, M.T. 1984. Acute effects of mitoxantrone on the template activity of isolated nuclei from the T-47D human breast tumor cell line. Cancer Lett. 21, 329-336
Darzynkiewicz, Z., Kapucinski, J., Carter, S.P., Schmid, F.A. and Melamed, M.R. 1986. Cytostatic and cytotoxic properties of pyronin Y: relation to mitochondrial localization of the dye and its interaction with RNA. Cancer Res., 46, 5760-576
de Clercq, E., Wells, R., Grant, R. and Merigan, T. 1971. Thermal activation of the antiviral activity of synthetic double-stranded polyribonucleotides. J. Mol. Biol. 56, 83-100
Fakan, S. and Puvion, E. 1980. The ultrastructural visualization of nucleolar and extranucleolar RNA synthesis and distribution. Int. Rev. Cytol., 65, 255-299
Gilloteaux, J., Jamison, J.M. and Summers, J.L. 1993. Subcellular distribution of an ethidium bromide/poly r(A-U) complex: confocal microscopy and ultrastructure. Scanning, 15, 55-56
Gilloteaux, J., Jamison, J.M., Zenhausern, F, Adrian, M. and Summers, J.L. 1997. Scanning force and transmission electron microscopy of poly r(A-U) and ethidium bromide-poly r(A-U) complexes. Scanning, 19, 523-532
Goessens, G. 1978. Nucleolar ultrastructure during reversible inhibition of RNA synthesis in chick fibroblasts cultivated in vitro. J. Ultrastruct Res., 65, 83-89
Goessens, G. 1979. Relations between fibrillar centres and nucleolus-associated chromatin in Ehrlich tumour cells. Cell Biol. Intl. Rep., 3, 337-343
Goessens, G. 1984. Nucleolar structure. Int. Rev. Cytol., 87, 107-158.
Hadjiolov, A.A. 1985. Ribosome biogenesis in the life cycle of normal and cancer cells. In: The nucleolus and ribosome biogenesis, cell biology monograph (ed. M. Alfert). Springer Verlag, Vienna, vol. 12, 165-196
Hobeika, A.C., Subramaniam, P.S. and Johnson, H.M. 1997. IFNα induces the expression of the cyclin-dependent kinase inhibitor p21 in human prostate cancer cells. Oncogene, 14, 1165-1170
Hubbell, H.R. 1986. Synergistic antiproliferative effect of human interferons in combination with mismatched double-stranded RNA on human tumor cells. Int. J. Cancer, 37, 359-365
Hubbell, H.R., Liu, R-S. and Maxwell, B.L. 1984. Independent sensitivity of human tumor cell lines to interferon and double-stranded RNA. Cancer Res., 44, 3252-3257
Iliescu, R., Repanovci, R., Mutiu, R., Sahnazarov, N., Danielescu, G., Popa, L.M. and Cajal, N. 1983. Investigations of the effect of cellular and viral nucleic acids on certain virus infections. Note 2. Effect of nucleic acids on virus multiplication in cell cultures. Rev. Roum. Med.-Virol., 34, 191-196.
Jamison, J.M., Krabill, K., Allen, K.A., Stuart, S.H. and Tsai, C-C. 1990. RNA-intercalative agent interactions: in vitro antiviral activity studies. Antiviral Chem. Chemother. 1, 333-347.
Jamison, J.M., Summers, J.L. and Gilloteaux, J. 1993a. Subcellular distribution of an ethidium bromide, poly r(A-U) complex. In: Proceedings of the 51st Annual Meeting of the Microscopy Society of America (eds G.W. Bailey and C.L. Reider). San Francisco Press, San Francisco, 196-197
Jamison, J.M., Gilloteaux, J. and Summers, J.L. 1993b. Effect of ethidium bromide on the morphology, antiviral activity and subcellular distribution of poly r(A-U). Cell Biol. Intl. Rep., 17, 1091-1105
Jamison, J.M., Gilloteaux, J. and Summers, J.L. 1994. Antiviral activity of RNA-dye combinations. In: Progress in molecular and subcellular biology. Biological response modifiers - interferons, (eds W.E.G. Müller and H.C. Schröder). Springer Verlag, Berlin vol. 14, 89-113
Jiang, H., Lin, J.J., Tao, J. and Fisher, P.B. 1997. Suppression of human ribosomal protein L23A expression during cell growth inhibition by interferon-β. Oncogene, 14, 473-480
Jocham, D., Beer, M., Baumgartner, R. Staehler, G. and Unsold, E. 1989. Long term experience with integral photodynamic therapy of TIS bladder carcinoma. Ciba Foundation Symposium, 146, 198-208
Jordan, E.G. and McGovern, J. 1981. The quantitative relationship of the fibrillar centres and other nucleolar components to changes in growth conditions, serum deprivation and low doses of actinomycin D in cultured diploid human fibroblasts (strain MRC-5). J. Cell Sci., 52, 373-389
Kapucinski, J. and Darzynkiewicz, Z. 1986. Relationship between the pharmacological activity of antitumor drugs ametantrone and mitoxantrone (novatrone) and their ability to condense nucleic acids. Proc. Natl. Acad. Sci. USA, 83, 6302-6306
Kapucinski, J. and Darzynkiewicz, Z., Traganos, F. and Melamed, M.R. 1981. Interactions of a new antitumor agent, 1, 4-dihydroxy-5,8-bis-[[2(-hydroxyethyl)amino]ethyl]amino]-9,10-anthracenedione, with nucleic acids. Biochem. Pharmacol., 30, 231-240
Kapucinski. J., Traganos, F. and Darzynkiewicz, Z. 1988. Interactions of antitumor drug ditercalinium with nucleic acids in solution and in situ. Cancer Lett., 42, 185-191
Kuhn, J.G., Balmer, C.E., Ludden, T.M., Loesch, D.M., Von Hoff, D.D., Bender, J.F. and Grillo-Lopez, A.J. 1987. Pharmacokinetics of ametantrone acetate (NSC-287513). Cancer Chemother. Pharmacol., 19, 133-137
Mahler, R.R. and Perlman, P.S. 1972. Effects of mutagenic treatment by ethidium bromide on cellular and mitochondrial phenotype. Arch. Biochem. Biophys., 148, 115-129
Marin, J.G., Rodrigue, J.M., Mencia-Huerta, P.E. and Braquet, P. 1993. Activation of the murine monocyte/macrophage cell line, J774A1 by poly (A); • poly (U) and induction of oligo-2′,5′-adenylate synthetase. Biochim. Biophys. Acta, 1178, 103-110
Medina, F., Risueno, M. and Diaz de la Espina, S. 1983. 3-D reconstruction and morphometry of fibrillar centres in plant cells in relation to nucleolar activity. Biol. Cell., 48, 31-38
Parker, S.L., Tong, T., Bolden, S. and Wingo, P.A. 1996. Cancer statistics, 1996. CA Cancer J. Clin., 65, 5-27
Prout, G.R., Jr., Lin, C-W., Benson, R.C., Nseyo, U.O., Daly, J.J., Griffin, P.P., Kinsey, J., Tian, M., Lao, Y., Mian, Y., Chen, X., Ren, F. and Qiao, S. 1987. Photodynamic therapy with hematoporphyrin derivative in the treatment of superficial transitional cell carcinoma of the bladder. N. Engl. J. Med., 317, 1251-1255
Puvion-Dutilleul, F., Mazan, S., Nicoloso, M., Pichard, E., Bachellerie, J.P. and Puvion, E. 1992. Alterations of nucleolar ultrastructure and ribosome biogenesis by actinomycin D. Implications for U3 snRNP function. Eur. J. Cell Biol., 58, 149-162
Raven, T., Hovanessian, A.T., Meurs, E.F., Sonenberg, N. and Kimchi, A. 1996. Double-stranded RNA-dependent protein kinase mediates c-myc suppression induced by type I interferons. J. Biol. Chem., 271, 25479-25484
Simard, R., Langelier, Y., Rosemonde, M., Maestracci, N. and Royal, A. 1974. Inhibitors as tools in elucidating the structure and function of the nucleus. In: The Cell Nucleus (ed. H. Busch). Academic Press, New York vol. 3, 447-487
Smetana, K. and Busch, H. 1974. The nucleolus and nucleolar DNA. In: The Cell Nucleus (ed. H. Busch). Academic Press, New York, vol. 1, 73-147
Soloway, M.S. 1988. The management of superficial bladder cancer, with emphasis on intravesical chemotherapy. In: The Management of Bladder Cancer (ed. D. Raghavan). Edward Arnold, London, 118-139
Stahl, A. 1982. The nucleolus and nucleolar chromosomes. In: The Nucleolus, Society for Experimental Biology, Seminar Series 15 (eds. E.G. Jordan and C.A. Cullis). Cambridge University Press, Cambridge, UK, 1-24
Thiry, M. 1992a. Highly sensitive immunodetection of DNA on sections with exogenous terminal deoxynucleotidyl transferase and non-isotopic nucleotide analogues. J. Histochem. Cytochem., 40, 411-419
Thiry, M. 1992b. New data concerning the functional organization of the mammalian cell nucleolus: detection of RNA and rRNA by in situ molecular immunocytochemistry. Nucleic Acids Res., 20, 6195-6200
Thiry, M., Jamison, J.M., Gilloteaux, J., Summers, J.L. and Goessens, G. 1997. Ultrastructural nucleolar alterations induced by an ametantrone/poly r(A-U) complex. Exp. Cell Res., 236, 275-284
Traganos, F. 1983. Dihydroxyanthraquinone and related bis(substituted) aminoanthraquinones: a novel class of antitumor agents. Pharmacol. Ther., 22, 199-214
Traganos, F., Bueti, C., Kapucinski, J. and Darzynkiewicz, Z. 1989. Antitumor intercalating drug ditercalinium binds preferentially to RNA in Friend erythroleukemia cells. Leukemia, 3, 522-529
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.
