A composite recombinant salivary proteins biomarker for Phlebotomus argentipes provides a surveillance tool post-elimination of visceral leishmaniasis in India.
[en] Incidence of visceral leishmaniasis (VL) in the Indian subcontinent (ISC) has declined by more than 95% since initiation of the elimination program in 2005. As the ISC transitions to the post-elimination surveillance phase, an accurate measurement of human-vector contact is needed to assure long-term success. To develop this tool, we identified PagSP02 and PagSP06 from saliva of Phlebotomus argentipes, the vector of Leishmania donovani in the ISC, as immunodominant proteins in humans. We also established the absence of cross-reactivity with Ph. papatasi saliva, the only other human-biting sand fly in the ISC. Importantly, by combining recombinant rPagSP02 and rPagSP06 we achieved greater antibody recognition and specificity than single salivary proteins. The Receiver Operating Characteristics curve for rPagSP02 + rPagSP06 predicts exposure to Ph. argentipes bites with 90% specificity and 87% sensitivity compared to negative control sera (P >0.0001). Overall, rPagSP02 + rPagSP06 provides an effective surveillance tool for monitoring vector control efforts post-VL elimination.
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Iniguez, Eva; Vector Molecular Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA
Saha, Samiran; Department of Biotechnology, Institute of Science, Visva Bharati University, West Bengal, India
Petrellis, Georgios ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) ; Vector Molecular Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA ; Laboratory of Microbiology, Parasitology and Hygiene, University of Antwerp, Antwerp, Belgium
Menenses, Claudio; Vector Molecular Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA
Herbert, Samantha; Vector Molecular Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA
Gonzalez-Rangel, Yvonne; Vector Molecular Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA
Rowland, Tobin; Entomology Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA
Aronson, Naomi E; Infectious Diseases Division, Department of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
Rose, Clair; Department of Parasitology and Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
Haines, Lee Rafuse; Department of Parasitology and Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
Acosta-Serrano, Alvaro; Department of Parasitology and Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
Serafim, Tiago D; Vector Molecular Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA
Oliveira, Fabiano ; Vector Molecular Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA
Srikantiah, Sridhar; Bihar Technical Support Program, CARE India Solutions for Sustainable Development, Patna, India
Bern, Caryn; Department of Epidemiology and Biostatistics, University of California, San Francisco, San Francisco, CA, USA
Valenzuela, Jesus G; Vector Molecular Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA
Kamhawi, Shaden; Vector Molecular Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA
A composite recombinant salivary proteins biomarker for Phlebotomus argentipes provides a surveillance tool post-elimination of visceral leishmaniasis in India.
NIH - National Institutes of Health [US-MD] [US-MD] NIAID - National Institute of Allergy and Infectious Diseases [US-MD] [US-MD] BMGF - Bill and Melinda Gates Foundation [US-WA] [US-WA] Global Emerging Infections Surveillance Section, Armed Forces Health Surveillance Branch, Department of Defense
