[en] Bacillus velezensis is considered as model species for plant-associated bacilli providing benefits to its host such as protection against phytopathogens. This is mainly due to the potential to secrete a wide range of secondary metabolites with specific and complementary bioactivities. This metabolite arsenal has been quite well defined genetically and chemically but much remains to be explored regarding how it is expressed under natural conditions and notably how it can be modulated upon interspecies interactions in the competitive rhizosphere niche. Here, we show that B. velezensis can mobilize a substantial part of its metabolome upon the perception of Pseudomonas, as a soil-dwelling competitor. This metabolite response reflects a multimodal defensive strategy as it includes polyketides and the bacteriocin amylocyclicin, with broad antibiotic activity, as well as surfactin lipopeptides, contributing to biofilm formation and enhanced motility. Furthermore, we identified the secondary Pseudomonas siderophore pyochelin as an info-chemical, which triggers this response via a mechanism independent of iron stress. We hypothesize that B. velezensis relies on such chelator sensing to accurately identify competitors, illustrating a new facet of siderophore-mediated interactions beyond the concept of competition for iron and siderophore piracy. This phenomenon may thus represent a new component of the microbial conversations driving the behavior of members of the rhizosphere community.
Disciplines :
Microbiology
Author, co-author :
Andric, Sofija ; Université de Liège - ULiège > Université de Liège - ULiège
Rigolet, Augustin ; Université de Liège - ULiège > TERRA Research Centre
Argüelles Arias, Anthony ; Microbial Processes and Interactions Laboratory, Terra Teaching and Research Center, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium
Steels, Sébastien ; Université de Liège - ULiège > Département GxABT > Microbial technologies
Hoff, Grégory ; Université de Liège - ULiège > Département GxABT > Microbial technologies ; Ecology and Biodiversity, Department of Biology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands
Balleux, Guillaume ; Université de Liège - ULiège > Département GxABT > Microbial technologies
Ongena, Loïc ; Université de Liège - ULiège > GIGA > GIGA Molecular Biology of Diseases - Gene Expression & Cancer
Höfte, Monica ; Laboratory of Phytopathology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium
Meyer, Thibault ; Université de Liège - ULiège > TERRA Research Centre > Microbial technologies ; UMR Ecologie Microbienne, F-69622, University of Lyon, Université Claude Bernard Lyon 1, CNRS, INRAE, VetAgro Sup, Villeurbanne, France. thibault.meyer@univ-lyon1.fr
Ongena, Marc ; Université de Liège - ULiège > Département GxABT
Language :
English
Title :
Plant-associated Bacillus mobilizes its secondary metabolites upon perception of the siderophore pyochelin produced by a Pseudomonas competitor.
This work was supported by the EU Interreg V France-Wallonie-Vlaanderen portfolio SmartBiocontrol (Bioscreen and Bioprotect projects, avec le soutien du Fonds européen de développement régional - Met steun van het Europees Fonds voor Regionale Ontwikkeling), the European Union Horizon 2020 research and innovation program under grant agreement No. 731077 and by the EOS project ID 30650620 from the FWO/F.R.S.-FNRS. AR and GB are recipient of a F.R.I.A. fellowship (F.R.S.-FNRS, National Funds for Scientific Research in Belgium) and MO is Research Director at the F.R.S.-FNRS.
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