LC-MS/MS; immunocapture; mass spectrometry; Biochemistry (medical); Clinical Biochemistry; General Medicine
Abstract :
[en] Immunocapture is now a well-established method for sample preparation prior to quantitation of peptides and proteins in complex matrices. This short review will give an overview of some clinical applications of immunocapture methods, as well as protocols with and without enzymatic digestion in a clinical context. The advantages and limitations of both approaches are discussed in detail. Challenges related to the choice of mass spectrometer are also discussed. Top-down, middle-down, and bottom-up approaches are discussed. Even though immunocapture has its limitations, its main advantage is that it provides an additional dimension of separation and/or isolation when working with peptides and proteins. Overall, this short review demonstrates the potential of such techniques in the field of proteomics-based clinical medicine and paves the way for better personalized medicine.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Massonnet, Philippe ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chimie clinique
Grifnee, Elodie ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chimie clinique
Farre Segura, Jordi ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Demeuse, Justine ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Huyghebaert, Loreen ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chimie clinique
Dubrowski, Thomas ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chimie clinique
Dufour, Patrice ; Centre Hospitalier Universitaire de Liège - CHU > > Pool assistant - biologie clinique
Schoumacher, Matthieu ; Université de Liège - ULiège > Unités de recherche interfacultaires > Centre Interdisciplinaire de Recherche sur le Médicament (CIRM)
Peeters, Stéphanie ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chimie clinique
Le Goff, Caroline ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chimie clinique
Cavalier, Etienne ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chimie clinique
Language :
English
Title :
Concise review on the combined use of immunocapture, mass spectrometry and liquid chromatography for clinical applications.
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