[en] Despite well-established pathways and metabolites involved in grapevine-Plasmopara viticola interaction, information on the molecules involved in the first moments of pathogen contact with the leaf surface and their specific location is still missing. To understand and localise these molecules, we analysed grapevine leaf discs infected with P. viticola with MSI. Plant material preparation was optimised, and different matrices and solvents were tested. Our data shows that trichomes hamper matrix deposition and the ion signal. Results show that putatively identified sucrose presents a higher accumulation and a non-homogeneous distribution in the infected leaf discs in comparison with the controls. This accumulation was mainly on the veins, leading to the hypothesis that sucrose metabolism is being manipulated by the development structures of P. viticola. Up to our knowledge this is the first time that the localisation of a putatively identified sucrose metabolite was shown to be associated to P. viticola infection sites.
Disciplines :
Chemistry
Author, co-author :
Maia, Marisa; Laboratório de FTICR e Espectrometria de Massa Estrutural, Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal ; Grapevine Pathogen Systems Lab (GPS Lab), Biosystems and Integrative Sciences Institute (BioISI), Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal
Mc Cann, Andréa ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Malherbe, Cédric ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Far, Johann ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Cunha, Jorge; Estação Vitivinícola Nacional, Instituto Nacional de Investigação Agrária e Veterinária (INIAV), Torres-Vedras, Portugal
Eiras-Dias, José; Estação Vitivinícola Nacional, Instituto Nacional de Investigação Agrária e Veterinária (INIAV), Torres-Vedras, Portugal
Cordeiro, Carlos; Laboratório de FTICR e Espectrometria de Massa Estrutural, Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal ; MARE - Marine and Environmental Sciences Centre, Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal
Eppe, Gauthier ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Quinton, Loïc ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie biologique
Figueiredo, Andreia; Grapevine Pathogen Systems Lab (GPS Lab), Biosystems and Integrative Sciences Institute (BioISI), Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal
De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences)
Sousa Silva, Marta; Laboratório de FTICR e Espectrometria de Massa Estrutural, Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal ; MARE - Marine and Environmental Sciences Centre, Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal
Language :
English
Title :
Grapevine leaf MALDI-MS imaging reveals the localisation of a putatively identified sucrose metabolite associated to Plasmopara viticola development.
The authors acknowledge the support from Fundação para a Ciência e a Tecnologia (Portugal) though the projects PTDC/BAA-MOL/28675/2017, UID/MULTI/04046/2019, UIDB/04292/2020, Investigator FCT programs IF 00819/2015 to Andreia Figueiredo and CEECIND/02246/2017 to Marta Sousa Silva and the PhD grant SFRH/BD/116900/2016 to Marisa Maia. Andréa McCann thank the Excellence of Science Program of the FNRS F.R.S (Rhizoclip EOS2018000802) for financial support. Cedric Malherbe acknowledge support from the F.R.S.-FNRS as Research Associate fellowship. The authors also acknowledge the support from the Portuguese Mass Spectrometry Network (LISBOA-01-0145-FEDER-022125) and the Project EU_FT-ICR_MS, funded by the Europe and Union’s Horizon 2020 research and innovation program under grant agreement nr.731077 (under a staff exchange program). The MALDI FT-ICR SolariX XR instrument was funded by FEDER BIOMED HUB Technology Support (number 2.2.1/996) and the SunChrom sprayer was founded by the European Union’s Horizon 2020 program [EURLipids Interreg Eurogio Meuse-Rhine project supported by the European Regional Development Fund (FEDER)].
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