[en] Acclimation to different light regimes is at the basis of survival for photosynthetic organisms, regardless of their evolutionary origin. Previous research efforts largely focused on acclimation events occurring at the level of the photosynthetic apparatus and often highlighted species-specific mechanisms. Here, we investigated the consequences of acclimation to different irradiances in Chlorella vulgaris, a green alga that is one of the most promising species for industrial application, focusing on both photosynthetic and mitochondrial activities. Moreover, proteomic analysis of cells acclimated to high light (HL) or low light (LL) allowed identification of the main targets of acclimation in terms of differentially expressed proteins. The results obtained demonstrate photosynthetic adaptation to HL versus LL that was only partially consistent with previous findings in Chlamydomonas reinhardtii, a model organism for green algae, but in many cases similar to vascular plant acclimation events. Increased mitochondrial respiration measured in HL-acclimated cells mainly relied on alternative oxidative pathway dissipating the excessive reducing power produced due to enhanced carbon flow. Finally, proteins involved in cell metabolism, intracellular transport, gene expression, and signaling-including a heliorhodopsin homolog-were identified as strongly differentially expressed in HL versus LL, suggesting their key roles in acclimation to different light regimes.
ERC - European Research Council [BE] PMI - Philip Morris International [CH] F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] ULiège - University of Liège [BE]
Funding text :
Philip Morris International funded the proteomics investigations. The research was supported by the H2020 European Research Council Starting Grant SOLENALGAE (679814) to MB. CR acknowledges Fonds De La Recherche Scientifique FNRS-FWO EOS Project 30829584, FNRS CDR J.0175.20, and Action de Recherche Concertée from the University of Liege (DARKMET ARC grant 17/21-08).
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