[en] A Bacillus subtilis derivative was obtained from strain ATCC 6633 by replacement of the native promoter (if he mycosubtilin operon by a constitutive promoter originating from the replication gene repU of the Staphylococcus aureus plasmid pUB110. The recombinant strain, designated BBG100, produced up to 15-fold more mycosubtilin than the wild type produced. The overproducing phenotype was related to enhancement of the antagonistic activities against several yeasts and pathogenic fungi. Hemolytic activities were also clearly increased in the modified strain. Mass spectrometry analyses of enriched mycosubtilin extracts showed similar patterns of lipopeptides for BBG100 and the wild type. Interestingly, these analyses also revealed a new form of mycosubtilin which was more easily detected in the BBG100 sample. When tested for its biocontrol potential, wild-type strain ATCC 6633 was almost ineffective for reducing a Pythium infection of tomato seedlings. However, treatment of seeds with the BBG100 overproducing strain resulted in a marked increase in the germination rate of seeds. This protective effect afforded by mycosubtilin overproduction was also visualized by the significantly greater fresh weight of emerging seedlings treated with BBG100 compared to controls or seedlings inoculated with the wild-type strain.
Disciplines :
Microbiology Biotechnology
Author, co-author :
Leclère, Valérie; University of Science and Technology of Lille > Laboratory of Microbial Bioprocesses, Polytech’Lille
Béchet, Max; University of Science and Technology of Lille > Laboratory of Microbial Bioprocesses, Polytech’Lille
Adam, Akram
Guez, Jean-Sébastien
Wathelet, Bernard ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Ongena, Marc ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Thonart, Philippe ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Gancel, Frédérique
Chollet-Imbert, Marlène
Jacques, Philippe ; Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Language :
English
Title :
Mycosubtilin overproduction by Bacillus subtilis BBG100 enhances the organism's antagonistic and biocontrol activities
Publication date :
August 2005
Journal title :
Applied and Environmental Microbiology
ISSN :
0099-2240
eISSN :
1098-5336
Publisher :
Amer Soc Microbiology, Washington, United States - Washington
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