[en] Aims: To compare the Belgian swabbing sampling method for pig carcasses
with the reference destructive method with regard to Escherichia coli and
aerobic plate counts, Salmonella and Campylobacter prevalence and their
relationship.
Methods and Results: Recovery was significantly lower for the swabbing
method and corresponded to a recovery of 36% for E. coli counts and 81% for
aerobic plate counts in comparison with the destructive method. There was no
significant difference between the swabbing and destructive sampling methods
for the prevalence of Salmonella or Campylobacter. A higher median for E. coli
counts was detected for samples where Salmonella or Campylobacter were
detected. The same association was also observed between the median for aerobic
plate counts and the presence of Campylobacter.
Conclusions: The method of swabbing used, covering 600 cm2 on each half-pig
carcass, is efficient for the sampling of pig carcasses in comparison with the
reference destructive method.
Significance and Impact of the Study: This study describes an efficient method
for microbiological pig carcass sampling. The Belgian swabbing method should
continue to be used to allow the follow up of bacterial contamination in the
Belgian meat production chain.
Disciplines :
Food science
Author, co-author :
Ghafir, Yasmine
Daube, Georges ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires
Language :
English
Title :
Comparison between swabbing and destructive methods for microbiological pig carcass sampling.
Publication date :
2008
Journal title :
Letters in Applied Microbiology
ISSN :
0266-8254
eISSN :
1472-765X
Publisher :
Blackwell Publishing
Volume :
47
Pages :
322-326
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
AFSCA - Agence Fédérale pour la Sécurité de la Chaîne Alimentaire
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Bibliography
Botteldoorn, N., Heyndrickx, M., Rijpens, N., Grijspeerdt, K. Herman, L. (2003) Salmonella on pig carcasses: positive pigs and cross contamination in the slaughterhouse. J Appl Microbiol 95, 891 903.
Byrne, B., Dunne, G., Lyng, J. Bolton, D.J. (2005) Microbiological carcass sampling methods to achieve compliance with 2001/471/EC and new hygiene regulations. Res Microbiol 156, 104 106.
Capita, R., Prieto, M. Alonso-Calleja, C. (2004) Sampling methods for microbiological analysis of red meat and poultry carcasses. J Food Prot 67, 1303 1308.
Churchill, G.A. Doerge, R.W. (1994) Empirical threshold values for quantitative trait mapping. Genetics 138, 963 971.
Davies, R.H., Dalziel, R., Gibbens, J.C., Wilesmith, J.W., Ryan, J.M.B., Evans, S.J., Byrne, C., Paiba, G.A. et al. (2004) National survey for Salmonella in pigs, cattle and sheep at slaughter in Great Britain (1999-2000). J Appl Microbiol 9, 750 760.
Dorsa, W.J., Cutter, C.N. Siragusa, G.R. (1996) Evaluation of six sampling methods for recovery of bacteria from beef carcass surfaces. Lett Appl Microbiol 22, 39 41.
Ducoffre, G. (2006) Surveillance des maladies infectieuses par un réseau de laboratoires de microbiologie 2005. Tendances épidémiologiques 1983-2004. Brussels : Scientific Institute of Public Health.
Eblen, D.R., Levine, P., Rose, B.E., Saini, P., Mageau, R. Hill, W.E. (2005) Nationwide microbiological baseline data collected by sponge sampling during 1997 and 1998 for cattle, swine, turkeys, and geese. J Food Prot 68, 1848 1852.
European Commission (2005) Commission Regulation (EC) no. 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs. Off J Eur Union 338, 1 26.
European Food Safety Authority (2006) The community summary report on trends and sources of zoonoses, zoonotic agents, antimicrobial resistance and foodborne outbreaks in the European Union in 2005. EFSA J 94, 1 236.
Fosse, J., Laroche, M., Rossero, A., Federighi, M., Seegers, H. Magras, C. (2006) Recovery methods for detection and quantification of Campylobacter depend on meat matrices and bacteriological or PCR tools. J Food Prot 69, 2100 2106.
Ghafir, Y., China, B., Korsak, N., Dierick, K., Collard, J.-M., Godard, C., De Zutter, L. Daube, G. (2005) Belgian surveillance plans to assess changes in Salmonella prevalence in meat at different production stages. J Food Prot 68, 2269 2277.
Ghafir, Y., China, B., Dierick, K., De Zutter, L. Daube, G. (2007) A seven-year survey of Campylobacter contamination in meat at different production stages in Belgium. Int J Food Microbiol 116, 111 120.
Ghafir, Y., China, B., Dierick, K., De Zutter, L. Daube, G. (2008) Hygiene indicator microorganisms for selected pathogens on beef, pork, and poultry meats in Belgium. J Food Prot 71, 35 45.
Gill, C.O. Jones, T. (2000) Microbiological sampling of carcasses by excision or swabbing. J Food Prot 63, 167 173.
Hutchison, M.L., Walters, L.D., Avery, S.M., Reid, C.A., Wilson, D., Howell, M., Johnston, A.M. Buncic, S. (2005) A comparison of wet-dry swabbing and excision sampling methods for microbiological testing of bovine, porcine, and ovine carcasses at red meat slaughterhouses. J Food Prot 68, 2155 2162.
ISO 17025 : 2005. General Requirements for the Competence of Testing and Calibration Laboratories. Geneva, Switzerland : International Organization for Standardization.
ISO 17604 : 2003. Microbiology of Food and Animal Feeding Stuffs - Carcass Sampling for Microbiological Analysis. Geneva, Switzerland : International Organization for Standardization.
Korsak, N., Daube, G., Ghafir, Y., Chahed, A., Jolly, S. Vindevogel, H. (1998) An efficient sampling technique used to detect four foodborne pathogens on pork and beef carcasses in nine Belgian abattoirs. J Food Prot 61, 535 541.
Lindblad, M., Lindmark, H., Lambertz, S.T. Lindqvist, R. (2007) Microbiological baseline study of swine carcasses at Swedish slaughterhouses. J Food Prot 70, 1790 1797.
Miraglia, D., Ranucci, D., D'Ovidio, V., Branciari, R. Severini, M. (2005) Comparison between carcass microbial load recovered by swabbing surfaces of different size and using the reference excision method. Vet Res Commun 29, 339 341.
National Reference Centre for Salmonella and Shigella (2006) Annual Report on Human Salmonella and Shigella in Belgium 2005. Brussels : Scientific Institute of Public Health.
Nesbakken, T., Eckner, K., Hoidal, H.K. Rotterud, O.J. (2003) Occurrence of Yersinia enterocolitica and Campylobacter spp. in slaughter pigs and consequences for meat inspection, slaughtering, and dressing procedures. Int J Food Microbiol 80, 231 240.
NF-V-08-051 : 1999. Microbiologie des aliments - Dénombrement des microorganismes par comptage des colonies obtenues à 30 degrés Celcius - Méthode de routine. La Plaine Saint-Denis, France : Association française de Normalisation.
Padungtod, P. Kaneene, J.B. (2005) Campylobacter in food animals and humans in northern Thailand. J Food Prot 68, 2519 2526.
Pearce, R.A. Bolton, D.J. (2005) Excision vs sponge swabbing - a comparison of methods for the microbiological sampling of beef, pork and lamb carcasses. J Appl Microbiol 98, 896 900.
Pepperell, R., Reid, C.A., Solano, S.N., Hutchison, M.L., Walters, L.D., Johnston, A.M. Buncic, S. (2005) Experimental comparison of excision and swabbing microbiological sampling methods for carcasses. J Food Prot 68, 2163 2168.
Phillips, D., Sumner, J., Alexander, J.F. Dutton, K.M. (2001) Microbiological quality of Australian beef. J Food Prot 64, 692 696.
Thorberg, B.M. Engvall, A. (2001) Incidence of Salmonella in five Swedish slaughterhouses. J Food Prot 64, 542 545.
United States Department of Agriculture (1995) Food safety and inspection service, Nationwide pork microbiological baseline data collection program: market hogs (April 1995-March 1996), Online at: http://www.fsis.usda.gov/ Science/Baseline_Data/index.asp.
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