Pharmacological Modulation of the Bystander Effect in the Herpes Simplex Virus Thymidine Kinase/Ganciclovir Gene Therapy System: Effects of Dibutyryl Adenosine 3',5'-Cyclic Monophosphate, Alpha-Glycyrrhetinic Acid, and Cytosine Arabinoside

Robe, Pierre; Princen, Frédéric; Martin, Didier; Malgrange, Brigitte; Stevenaert, Achille; Moonen, Gustave; Gielen, Jean-Louis; Merville, Marie-Paule; Bours, Vincent

doi:10.1016/S0006-2952(00)00315-4

Article (Scientific journals)

Pharmacological Modulation of the Bystander Effect in the Herpes Simplex Virus Thymidine Kinase/Ganciclovir Gene Therapy System: Effects of Dibutyryl Adenosine 3',5'-Cyclic Monophosphate, Alpha-Glycyrrhetinic Acid, and Cytosine Arabinoside

Robe, Pierre; Princen, Frédéric; Martin, Didier et al.

2000 • In Biochemical Pharmacology, 60 (2), p. 241-9

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/7199

DOI
10.1016/S0006-2952(00)00315-4

PubMed
10825469

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Keywords :

Animals; Antimetabolites, Antineoplastic/pharmacology; Antineoplastic Agents/administration & dosage/pharmacology; Bucladesine/pharmacology; Connexin 43/biosynthesis/genetics/metabolism; Connexins/biosynthesis; Cytarabine/pharmacology; Drug Screening Assays, Antitumor; Ganciclovir/administration & dosage/pharmacology; Gap Junctions/drug effects; Gene Therapy; Glioblastoma; Glycyrrhetinic Acid/pharmacology; Phosphorylation; RNA, Messenger/biosynthesis; Rats; Simplexvirus/enzymology; Thymidine Kinase/genetics/metabolism; Transfection; Tumor Cells, Cultured

Abstract :

[en] The herpes simplex virus type 1 thymidine kinase (HSV1-tk) suicide gene/ganciclovir system was first applied to the treatment of glioblastoma tumors, but was hampered by the low gene transfection yield. Fortunately, the gap junction-dependent diffusion of phosphorylated ganciclovir metabolites from transfected cells to their neighbors proved to enhance the overall benefit of this strategy. However, as tumor cells are often gap junction-deficient, we sought to restore this property pharmacologically and hence to improve the efficacy of the treatment. We demonstrated that this approach was feasible in glioblastoma cells using dibutyryl adenosine 3',5'-cyclic monophosphate (cAMP) (100 microM) as a pharmacological inducer of gap junctions. alpha-Glycyrrhetinic acid (25 microM), on the other hand, strongly inhibited both gap junction-mediated intercellular communication and the bystander effect, thus confirming the role of gap junctions in HSV-tk-mediated bystander killing. Using cytosine arabinoside as a growth inhibitor, we underlined the role of tumor cell proliferation in the sensitivity of HSV-tk-transfected cells to ganciclovir and demonstrated its correlation with the importance of the bystander effect.

Disciplines :

Surgery

Author, co-author :

Robe, Pierre ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques

Princen, Frédéric

Martin, Didier ; Université de Liège - ULiège > Département des sciences cliniques > Neurochirurgie

Malgrange, Brigitte ; Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.

Stevenaert, Achille ; Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)

Moonen, Gustave ; Université de Liège - ULiège > Département des sciences cliniques > Neurologie - Doyen de la Faculté de Médecine

Gielen, Jean-Louis ; Centre Hospitalier Universitaire de Liège - CHU > Chirurgie maxillo-faciale et plastique

Merville, Marie-Paule ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale

Bours, Vincent ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Génétique générale et humaine

Language :

English

Title :

Publication date :

15 July 2000

Journal title :

Biochemical Pharmacology

ISSN :

0006-2952

eISSN :

1873-2968

Publisher :

Elsevier, Netherlands

Volume :

Issue :

Pages :

241-9

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

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